Daunorubicin Liposomes (DaunoXome) within Tumor Tissue

Unilamellar liposomes that retain their contents in the systemic circu lation can alter the pharmacokinetics of anticancer agents in favorable ways. It has long been recognized that certain liposome compositions will extravasate at sites of growing tumors and may increase the local drug concentration substantially above that achievable with a free drug. We report here that liposomes can alter the in vivo disposition of an entrapped drug not only on a macroscopic but also on a microscopic scale. We show through in vitro studies that intact liposomes composed of distearoylphosphatidylcholine and cholesterol and containing daunorubicin (Dauno Xome) are taken up into P179S tumor cells. These liposomes produce an enhanced cytotoxicity relative to the free drug for incubation times longer than about 8 h. For in vivo studies, we developed and used a noninvasive fluorescence imaging technique to follow the accumulation of liposomal daunorubicin within murine tumors. With this method, we show that the maximum concentration of the available liposomal drug in tumors exceeds that of the free drug, and additionally, liposomal daunorubicin persists at high levels for several days. Total liposome-delivered drug fluorescence from whole tumor extracts peaks at about 8 h. In comparison, the fluo rescence intensity of daunorubicin released from vesicles seen with the in vivo imaging experiment peaks at 28-32 h. This apparent delay is due to a sustained release of the drug from liposomes in the tumor. Fluorescence microscopy of thin sections of tumors from animals injected i.v. with liposomal daunorubicin demonstrate persistent high levels of daunorubi cin fluorescence within cells and throughout the tumor masses. Free daunorubicin, in contrast, transiently achieves modest levels of fluores cence and rapidly drops to background within a few h. These results indicate distinct mechanisms for the localization of free and liposomal daunorubicin, suggesting that liposomal daunorubicin can provide sus tained intracellular levels of the drug within the tumor.